Yeast does not contribute to beer colour directly, other than colour loss during production via adsorption of coloured materials to their cell wall. Indirectly, yeast can affect beer colour via turbidity in products where it remains present at packaging, such as with hefeweizen.
Barley contains very low concentrations of pigmented substances, and it is the malting process that results in colour formation. The germination and kilning phases of the malting process set the stage for and determine the extent of colour formation from Maillard browning reactions and in some cases caramelization and pyrolysis reactions. Secondary to these heat driven colour-forming reactions, the oxidation of polyphenols derived from barley husk or hop vegetative matter can contribute to the colour formation during beer storage/ageing. Additionally, oxidation of polyphenols can lead to enhanced protein–polyphenol interaction and the formation of non-biological haze. The scattering of light via this haze indirectly affects a consumer’s perception of colour as well as its physical measurement.
Colour is a human visual perception utilising a narrow portion of the electromagnetic spectrum (380–780 nanometers). Light itself has no colour and colour does not exist by itself, it only exists in the mind of the viewer. Colour perception exists in two stages. The first is a purely physical phenomenon that requires three elements: a source of light, an object and a detector (an eye, a diode, etc.) while the second stage is a complicated and incompletely known process whereby the human eye transmits information that the brain will interpret as colour.
The principal attributes of any object’s colour are hue, lightness and saturation.
1. Hue is the quality we normally identify as an object’s colour, such as red, green, yellow and so forth. Hues form what we describe as the colour wheel. The human eye can identify more than a million different hues.
2. Lightness is a term related to the concept of light and dark and is used to classify colours by separating those that are bright, mid-tone or dark.
3. Saturation or chroma is completely separate from hue and lightness. It can be defined as the purity of colour; that is as a colour moves away from a central neutral Gray its saturation increases as it becomes more vivid and less dull.
Often these three colour attributes are described.
What is Light?
Primary Source
1) Directly from a light source.
Secondary Source
2) Light reflected off on object
3) Light transmission through the object.
For Secondary Sources, Interaction of Primary Light and Secondary Source is Very Important
In case of beer white light is transformed into yellow and blue when absorbed.
Measuring Beer Colour
Historically, beer colour was determined by visual comparisons against a set of colour standards. Joseph Lovibond developed a set of standards and a tintometer in 1893 while solutions of potassium chromate have been used as reference standards in the early 20th century. The use of standardised Lovibond coloured glasses made it possible to compare measurements from different Lovibond Tintometers. After a series of modifications, these standardised comparator discs were accepted by the European Brewing Convention in 1951. So ingrained was this approach that many brewers and maltsters today still refer to colour values in degrees Lovibond. The tintometer approach, while satisfactory at the time, had inherent flaws due to variation in colour perception by the human observer. Because red-green colour blindness is present at roughly 7% of the male population. Furthermore, instability of the glass or liquid colour standards over time, for instance, orange dichromate slowly reducing to green chromium, would lead to false colour estimates.
The solution to both of these issues was single wavelength measurements using a precision spectrophotometer.
Beer colour is measured in the Standard Reference Method (SRM) or European Brewery Convention (EBC) Units. SRM is based on the Lovibond scale and determined from a wort analysis. this is a spectrophotometer measurement, does not indicate a hue, only depth when viewed at a wavelength of 475 nanometers.
White shade of 2 SRM
Maillard browning reactions are key to beer colour and they originate in the barley endosperm during malting but can resume during wort boiling. The two key components of this reaction are reducing sugars (principally maltose) and free amino acids or amino groups of amino acids that comprise protein.
Colors produced by melanoidins are yellow, orange and red initially and turn to brown as the Maillard reaction is allowed to proceed. Lightly kilned malt displays yellow colors characteristic of light lager beer while intensely kilned products display amber and brown hues characteristic of British ales or Vienna lagers.
Colouring agents such as malt extracts and caramel colouring can be added post-fermentation as a means of adjusting the beer’s final colour. These products are intensely coloured with colours ranging from 250 to 3500 ° SRM for concentrated malt extracts and 5000 to 30,000 ° SRM for caramels.
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